DNA

Fun with Science and Grownups at NIH

Bioinformatician Keri Richards shows 7-year-old James vials from a freezer in her lab at NIDCD. The vials contain DNA samples.

Hi, my name is James. I’ve always really liked science and I want to be a scientist when I grow up, but I never got to see where scientists work until I went to Take Your Child to Work Day at NIH with my Auntie Kit. It was awesome!

Sliding Through Science History, Part 2

Dietzgen Binary Slide Rule

Are you beginning to think that slide rules look alike? If you could see the types and number of scales, you’d understand that each slide rule model is different. There are specialized scales for cubes, spheres, voltage, etc. Check out a few of the slide rules that made history with IRP investigators.

IRP Resources Pop Quiz: What Can We Do for Your Science?

As an “I Am Intramural” Blog reader, you likely know that the IRP is comprised of more than 6,000 scientists conducting basic, translational, and clinical research in more than 50 buildings on six different IRP campuses around the U.S.But, do you know the answers to the questions in the following IRP Pop Quiz?

Researchers at the NIH IRP have access to:

a) Laboratory equipment sales, rental, repairs, and maintenance
b) Plants and marine organisms for research
c) High-throughput DNA sequencing
d) A and C
e) All of the above

Learn the answer...

Bugs, Recycling, and DNA—Oh My!

Most workplaces would never think of having hawks, turtles, beetles and stick bugs at an event for kids—but most workplaces are not the National Institutes of Health. Each year, the NIH Bethesda campus holds its Earth Day celebration in conjunction with Take Your Child to Work Day. Employees share their love of science with their kids while also learning about how to protect the environment.

Read more...

Next Big Thing in Genome Modification: the CRISPR/Cas9 System

Ever since the discovery of the double-helix structure of DNA, scientists have sought ways to edit the genome. Altering gene expression partially and transiently via small interfering RNA has come a long way, and the progress has been spectacular. However, achieving complete and sustained modification of gene expression in a cell remains a tedious procedure that is often costly and time-consuming. For molecular biologists working with cell lines, quick and efficient knock out of one or more genes would provide a powerful tool for their studies. The CRISPR technology arrived two years ago to potentially fulfill that need.

CRISPR genome editing

For the Love of PCR

You mix everything together that’s necessary for the reaction, and half the time it works, half the time it doesn’t. One day you get great PCR results, you’re on cloud nine, everything worked, and then you go repeat it to verify the result (because n never equals 1 in science), and it doesn’t work. You begin to feel like maybe you just got lucky with the first experiment.

PCR curve

Breaking the Chains Forged in Life

In Charles Dickens’ 1843 classic, A Christmas Carol, Ebenezer Scrooge is visited by four ghosts who help him to see the error of his ways and embrace a life of service. Scrooge is then able to correct the actions that could have led to his demise. Researchers studying epigenetics take on a similar task.

Marley's Ghost

Why Studying Bacteria Matters

I have been thinking a lot recently about how the tools we use in our work have improved so dramatically in the last few decades and how this is mostly down to the frequently disparaged study of microbes. While everyone can get behind studying bacteria that cause life-threatening diseases like typhoid fever and cholera, I think that it is often harder to convince people of the value of studying ordinary and sometimes obscure bacteria that do not directly affect human health. However, over the years, such studies have revolutionized many aspects of our lives.

thermophillic bacteria at Yellowstone

Prevailing in Science

Ever since studying transposons (mobile genetic elements) in graduate school, I’ve been fascinated by DNA and the many natural ways DNA moves and recombines within genomes. Transposons are responsible for multidrug resistance in bacteria, and the major players in V(D)J recombination in humans were derived from transposons. Now, as a postdoctoral fellow in the National Cancer Institute of the NIH, I conduct research focused on gene therapy strategies for hematologic malignancies and immunodeficiencies, because I am interested in the clinical application of basic biology.

Jeanelle Spencer in the lab