How Asking a Very Basic Research Question Led Us to a Model For At Least 3 Diseases

By Howard Young

Monday, January 12, 2015

When I started this project, it was not my objective to develop a model for any specific disease, nor did I even suspect that the ultimate result would be some insight into autoimmune disease. The basic research question I was asking was why there are sequences in the 3’ untranslated region of the interferon-gamma mRNA that are more highly conserved than the coding region of the gene.

If one looks at the mRNA sequence of the Interferon-gamma gene from just about any species, from white rhinoceros to badger to humans (and even to some extent in the whale shark), this 3’UTR element is strongly conserved, thus indicating that it has an important role in regulating Interferon-gamma gene expression. It turns out that this region is enriched in AUUA sequences that make up what is termed the ARE (AU-Rich Element). At least a third of genes have such an element, and this region has been shown to mediate destruction of the mRNA through interaction with different RNA binding proteins. Thus, the conservation of this element indicates that rapid destruction of the IFN-gamma mRNA is an important mechanism for controlling IFN-gamma expression that has been retained throughout evolution.

With the help of many colleagues and lab members in the NCI, we generated a mouse where 162 nucleotides in the 3’ UTR, containing the ARE, were replaced with random sequences. The outcome of this genetic change was an IFN-gamma mRNA that was much more stable, resulting in constitutive expression of IFN-gamma mRNA in many tissues and a chronic, albeit low, expression of IFN-gamma protein in the serum.

Studies on these mice are ongoing, but we have observed that in the C57BL/6 strain of these mice, they develop a lupus–like syndrome by 10-12 weeks of age. In addition, we have recently learned that they also show evidence of primary biliary cirrhosis with a female bias, consistent to what is observed in humans (90% of cases are in women). Previous mouse models of PBC did not show a sex bias and in collaboration with an extramural lab that specializes in the human disease (Dr. M. Eric Gershwin, UC-Davis), we are working on understanding the mechanism involved in disease initiation and progression.

Finally, when we eliminated the ARE element and crossed the mice onto the Balb/c genetic background, we found evidence for the development of aplastic anemia, a disease whose connection to IFN-gamma had been shown for some time by the lab of Neal Young in the NHLBI. Furthermore, using an antibody that neutralizes IFN-gamma we were able to neutralize some of the effects observed in the mouse bone marrow, thus suggesting that targeting IFN-gamma may be worthy of consideration as a potential treatment for this disease.

I strongly believe that this project, based on a very basic research question stimulated by the support and encouragement of the NIH intramural program, reinforces the value of basic research. It demonstrates that the outcome of asking such questions cannot be predicted in advance, but may well lead to a better understanding of human disease.

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