Wednesday, January 2, 2019
The waning weeks of 2018 were busy ones in the Office of NIH History. We're constantly receiving and cataloguing new donations of historic equipment, images, publications, and more. It’s time to see what our donors have given us lately!
"I thought why could you not invert the concept? Instead of laying down hundreds or thousands of probes, how about laying down hundreds or thousands of tissue spots and probing them one antibody or gene probe at a time," remembers Dr. Juha Kononen of the National Human Genome Research Institute (NHGRI) about his idea that led to this prototype manual microarray. Tissue array technology performs rapid molecular profiling of hundreds of normal and pathological tissue specimens or cultured cells. Dr. Kononen worked with Drs. Olli Kallioniemi and Stephen B. Leighton to design this tissue microarray which was initially used in the Cancer Genomics Branch. Now, the National Cancer Institute's Tissue Array Research Program (TARP) develops and distributes multi-tumor tissue microarray slides to cancer researchers based on this technology. The quote comes from a 2002 article published in The Scientist.
Friday, March 13, 2015
Each day, hundreds of thousands of biomedical researchers around the world design and execute studies, with diverse trajectories and outcomes and where success is based largely on reproducibility. However, a large percentage of experiments using cell culture techniques have been labelled as irreproducible, with around 25 percent of all cell-line research described as either contaminated with other cells or mischaracterized in some way. In other words, if your kidney cancer cell isn’t really a kidney cancer line, then how will anyone else be able to reproduce your work?