Yun-Bo Shi, Ph.D.
Section on Molecular Morphogenesis
Thyroid Hormone Regulation of Vertebrate Postembryonic Development
We investigate the molecular mechanisms of thyroid hormone function during postembryonic development. The main model is the metamorphosis of the amphibians Xenopus laevis, a species well studied as a developmental model, and Xenopus troplicalis, a species more recently developed as a model system and whose shorter life cycle and sequenced genome offers some unique advantages. The control of postembryonic development by thyroid hormone (TH) offers a unique paradigm to study gene function in postembryonic organ development. During metamorphosis, various organs undergo vastly different changes. Some, such as the tail, undergo complete resorption while others, such as the limb, are developed de novo. The majority of the larval organs persist through metamorphosis but are dramatically remodeled to function in a frog. For example, tadpole intestine in Xenopus is a simple tubular structure consisting primarily of a single layer of larval epithelial cells. During metamorphosis, tadpole intestine is transformed into an organ with a multiply folded adult epithelium surrounded by elaborate connective tissue and muscles; the process involves specific larval epithelial cell death and de novo development of adult epithelial stem cells, which then proliferate and differentiate. The wealth of knowledge from earlier research and the ability to manipulate amphibian metamorphosis both in vivo, by using transgenesis or hormone treatment of whole animals, and in vitro in organ cultures offer an excellent opportunity to study the developmental function of TH receptors (TRs) and underlying mechanisms in vivo and to identify and functionally characterize genes that are critical for postembryonic organ development in vertebrates. Our studies have revealed likely conserved mechanisms of adult intestinal stem cell development in vertebrates, prompting us to adapt the mouse model, which complements the amphibian system, in which gene knockout is not yet available.
Dr. Yun-Bo Shi is a senior investigator and the head of Section on Molecular Morphogenesis, Laboratory of Gene Regulation and Development, Program on Cell Regulation and Metabolism, at NICHD. Dr. Shi received his B. S. degree from the Department of Chemistry, Wuhan University, China, in 1982 and his Ph. D. degree from the Department of Chemistry, University of California, Berkeley, in 1988. After postdoctoral training at the Carnegie Institution, Baltimore, MD, Dr. Shi established his own research group within the intramural research program of NICHD in 1992.
His laboratory has been studying the molecular basis of thyroid hormone regulation of vertebrate development by using Xenopus metamorphosis as a model system. Dr. Shi has published over 160 research papers and reviews/book chapters, edited two books, and written a monograph on amphibian metamorphosis. For his accomplishments, Dr. Shi has received many awards, including the 2009 NIH Asian & Pacific Islander American Organization Award for outstanding accomplishments in biomedical research and the 2008 Van Meter Award by the American Thyroid Association, which honors an investigator who has made outstanding contributions to research on the thyroid gland. Dr. Shi is a board member of several journals including Thyroid, Molecular Endocrinology, and the Journal of Biological Chemistry. Dr. Shi served as an editor of Cell Research from 1997-2010 and is currently the Editor-in-Chief of Cell & Bioscience.
Wen L, Fu L, Guo X, Chen Y, Shi YB. Histone methyltransferase Dot1L plays a role in postembryonic development in Xenopus tropicalis. FASEB J. 2015;29(2):385-93.
Heimeier RA, Das B, Buchholz DR, Fiorentino M, Shi YB. Studies on Xenopus laevis intestine reveal biological pathways underlying vertebrate gut adaptation from embryo to adult. Genome Biol. 2010;11(5):R55.
Matsuda H, Shi YB. An essential and evolutionarily conserved role of protein arginine methyltransferase 1 for adult intestinal stem cells during postembryonic development. Stem Cells. 2010;28(11):2073-83.
Wen L, Shibata Y, Su D, Fu L, Luu N, Shi YB. Thyroid Hormone Receptor α Controls Developmental Timing and Regulates the Rate and Coordination of Tissue-Specific Metamorphosis in Xenopus tropicalis. Endocrinology. 2017;158(6):1985-1998.
Okada M, Miller TC, Wen L, Shi YB. A balance of Mad and Myc expression dictates larval cell apoptosis and adult stem cell development during Xenopus intestinal metamorphosis. Cell Death Dis. 2017;8(5):e2787.
Related Scientific Focus Areas
Molecular Biology and Biochemistry
This page was last updated on July 12th, 2017