The goal of my lab’s research program is to provide basic science information that will lead to the development of more effective vaccines and drugs against bacterial infections.
Our research program focuses on the structure determination of integral membrane proteins. We perform cryo electron microscopy and X-ray crystallography together with functional analysis of these proteins using biophysical, biochemical, and cell biological techniques. We study transporters embedded in the outer membranes of Gram-negative bacteria, which are surface accessible and therefore have the potential to be good vaccine and/or drug targets against infectious diseases. We also study the membrane associated or soluble protein partners that interact with outer membrane transporters to understand how these systems function in vivo. Current topics in the lab include (1) small molecule and protein import across the bacterial outer membrane, (2) protein secretion by pathogenic bacteria, and (3) protein import across mitochondrial outer membranes.
Applying Our Research
This work advances medical research and will directly benefit the public. We have already succeeded in identifying an alternative drug treatment to antibiotics that may result in reduced multi-drug resistance in bacterial pathogens. We have also spent several years developing novel vaccines against plague. The approaches we use can be similarly applied to other bacterial diseases.
Need for Further Study
Multidrug resistance arising from indiscriminate and widespread use of antibiotics is a growing health threat, and we need to develop much better strategies for treatment and prevention of bacterial infections.
- Ph.D., Frankfurt University, 1990
- M.S., Princeton University, 1986
- B.S., Furman University, 1983
- Noinaj N, Easley NC, Oke M, Mizuno N, Gumbart J, Boura E, Steere AN, Zak O, Aisen P, Tajkhorshid E, Evans RW, Gorringe AR, Mason AB, Steven AC, Buchanan SK. Structural basis for iron piracy by pathogenic Neisseria. Nature. 2012;483(7387):53-8.
- Noinaj N, Kuszak AJ, Gumbart JC, Lukacik P, Chang H, Easley NC, Lithgow T, Buchanan SK. Structural insight into the biogenesis of β-barrel membrane proteins. Nature. 2013;501(7467):385-90.
- Celia H, Noinaj N, Zakharov SD, Bordignon E, Botos I, Santamaria M, Barnard TJ, Cramer WA, Lloubes R, Buchanan SK. Structural insight into the role of the Ton complex in energy transduction. Nature. 2016;538(7623):60-65.
- Celia H, Botos I, Ni X, Fox T, De Val N, Lloubes R, Jiang J, Buchanan SK. Cryo-EM structure of the bacterial Ton motor subcomplex ExbB-ExbD provides information on structure and stoichiometry. Commun Biol. 2019;2:358.
- Diederichs KA, Ni X, Rollauer SE, Botos I, Tan X, King MS, Kunji ERS, Jiang J, Buchanan SK. Structural insight into mitochondrial β-barrel outer membrane protein biogenesis. Nat Commun. 2020;11(1):3290.
Related Scientific Focus Areas
Microbiology and Infectious Diseases
Molecular Biology and Biochemistry
This page was last updated on Thursday, November 30, 2023