Skip to main content
 

Sergey M. Bezrukov, Ph.D.

Senior Investigator

Section on Molecular Transport

NICHD

Building 9, Room 1N124
9 Memorial Drive
Bethesda, MD 20892

301-402-4701

bezrukov@helix.nih.gov

Research Topics

Biophysics of Large Membrane Channels

We study mechanisms of transporter-facilitated metabolite exchange across cell and organelle membranes by combining physical theory and experiments on reconstitution of the corresponding “large” ion channels into planar lipid membranes. It is well-recognized that disturbances in metabolite transport result in a wide variety of pathologies in development and in different organ systems, including, among others, cancer, cystic fibrosis, seizure disorders, muscular dystrophies, and cardiomyopathies. Understanding of the physical mechanisms that govern metabolite transport is essential for creating new approaches to treatment of these pathologies.

To investigate channels under precisely controlled conditions, we first isolate the channel-forming proteins from their host organisms, then purify and reconstitute them into planar lipid membranes. Our main goal is to elucidate the physical principles and molecular mechanisms responsible for metabolite flux regulation under normal and pathological conditions. The channel-forming proteins of interest include the voltage-dependent anion channel from the outer membrane of mitochondria (VDAC), B-components of Clostridium botulinum C2 and Clostridium perfringens iota toxins, Bacillus anthracis protective antigen, Clostridium perfringens epsilon toxin, Escherichia coli general porin OmpF and sugar-specific LamB, Staphylococcus aureus alpha-hemolysin, Pseudomonas aeruginosa OprF, Trichoderma viride alamethicin, and Pseudomonas syringae lipopeptide toxin syringomycin E. We also use Bacillus brevis gramicidin A as a tool to study mechanical properties of lipid membranes.

Biography

Dr. Sergey Michael Bezrukov received his M.S. in physics from St. Petersburg Polytechnic University, Russia, his Ph.D. in biophysics from Moscow State University, and a D.Sci. in physics and mathematics from the Russian Academy of Sciences. He started his career as a researcher at the St. Petersburg Nuclear Physics Institute in Russia and moved to United States in 1990, first as a visiting research professor at the University of Maryland. In 1992, he joined the National Institutes of Health as a visiting scientist. He was awarded tenure by NIH in 2002. Dr. Bezrukov has authored numerous scientific papers, five of them published in Nature (London). He has organized and chaired many international meetings and workshops. Dr. Bezrukov’s recent honors include election to Fellowship in the American Physical Society (2009) and the NIH Director’s Award in Science and Medicine (2010). Dr. Bezrukov is the Chief of the Section on Molecular Transport, Program in Physical Biology, NICHD/NIH, a position he has held since October 2002. Dr. Bezrukov’s section combines physical theory with experiments on bacterial, mitochondrial, and toxin-induced membrane channels, reconstituted in planar lipid bilayers in order to address fundamental questions of membrane transport. This line of research serves as the basis for the development of new approaches to treatment of many diseases where regulation of transport through ion channels plays the key role.

Selected Publications

  1. Rostovtseva TK, Gurnev PA, Protchenko O, Hoogerheide DP, Yap TL, Philpott CC, Lee JC, Bezrukov SM. α-Synuclein Shows High Affinity Interaction with Voltage-dependent Anion Channel, Suggesting Mechanisms of Mitochondrial Regulation and Toxicity in Parkinson Disease. J Biol Chem. 2015;290(30):18467-77.
  2. Berezhkovskii AM, Dagdug L, Bezrukov SM. Discriminating between anomalous diffusion and transient behavior in microheterogeneous environments. Biophys J. 2014;106(2):L09-11.
  3. Gurnev PA, Rostovtseva TK, Bezrukov SM. Tubulin-blocked state of VDAC studied by polymer and ATP partitioning. FEBS Lett. 2011;585(14):2363-6.
  4. Sheldon KL, Maldonado EN, Lemasters JJ, Rostovtseva TK, Bezrukov SM. Phosphorylation of voltage-dependent anion channel by serine/threonine kinases governs its interaction with tubulin. PLoS One. 2011;6(10):e25539.
This page was last updated on October 18th, 2011