Robin Stanley, Ph.D.

Stadtman Investigator

Signal Transduction Laboratory/Nucleolar Integrity Group

NIEHS

F378 Rall Building
111 T W Alexander Dr
Research Triangle Park, NC 27709

984-287-3568

robin.stanley@nih.gov

Research Topics

Eukaryotic ribosome biogenesis is a complex process that involves the assembly of 79 ribosomal proteins with 4 ribosomal RNAs through the concerted effort of more than 200 non-ribosomal biogenesis factors. Dysfunction of this pathway gives rise to a group of human diseases known as ribosomopathies and deregulation of the pathway had been linked with numerous types of human cancers, underscoring the need to further understand this complex process. Ribosome biogenesis is one of the most energetically costly endeavors for a cell, at times using up to 80 percent of cellular energy resources. As a result, the process needs to be tightly regulated with cell cycle progression and environmental stimuli. The Stanley research group utilizes a multidisciplinary approach by combining structural biology (x-ray crystallography, electron microscopy, and small angle x-ray scattering) with biochemical and cell biology methods to understand 1) How ribosome assembly factors regulate ribosome assembly and 2) How cell cycle, environmental stimuli, and stress modulate ribosome production.

Biography

Robin Stanley, Ph.D., heads the Nucleolar Integrity Group within the NIEHS Signal Transduction Laboratory. Stanley is an Earl Stadtman Tenure-Track Investigator and earned her B.S. in chemistry and B.A. in mathematics from the University of North Carolina at Charlotte. She earned her Ph.D. in molecular biophysics and biochemistry at Yale University, where she worked under Nobel Laureate Thomas Steitz, Ph.D. Before joining NIEHS in 2014, she was a postdoctoral fellow with James H. Hurley, Ph.D., at the National Institute of Diabetes, Digestive, and Kidney Diseases.

Selected Publications

  1. Hayne CK, Schmidt CA, Haque MI, Matera AG, Stanley RE. Reconstitution of the human tRNA splicing endonuclease complex: insight into the regulation of pre-tRNA cleavage. Nucleic Acids Res. 2020;48(14):7609-7622.

  2. Lo YH, Sobhany M, Hsu AL, Ford BL, Krahn JM, Borgnia MJ, Stanley RE. Cryo-EM structure of the essential ribosome assembly AAA-ATPase Rix7. Nat Commun. 2019;10(1):513.

  3. Gordon J, Pillon MC, Stanley RE. Nol9 Is a Spatial Regulator for the Human ITS2 Pre-rRNA Endonuclease-Kinase Complex. J Mol Biol. 2019;431(19):3771-3786.

  4. Pillon MC, Hsu AL, Krahn JM, Williams JG, Goslen KH, Sobhany M, Borgnia MJ, Stanley RE. Cryo-EM reveals active site coordination within a multienzyme pre-rRNA processing complex. Nat Struct Mol Biol. 2019;26(9):830-839.

  5. Pillon MC, Sobhany M, Borgnia MJ, Williams JG, Stanley RE. Grc3 programs the essential endoribonuclease Las1 for specific RNA cleavage. Proc Natl Acad Sci U S A. 2017;114(28):E5530-E5538.


This page was last updated on August 19th, 2020