Quan Wang, Ph.D.
Section of Nanoscale Single-Molecule Dynamics, Laboratory of Chemical Physics
We are currently developing new and improved methods that expand the capability of single-molecule fluorescence spectroscopy in solution. Building on a platform technology known as anti-Brownian Electrokinetic (ABEL) trapping to control a single biomolecule in solution, we simultaneously measure single-molecular size, charge (Nat. Methods 11, 555) and conformations (Nat. Methods 18, 816), together with their time-dependent dynamics in real time. These capabilities provide rich quantitative information on the oligomerization, phosphorylation and structural states and state transitions on a single biomolecule, and serve as a unique observation window into a wide range of biological processes.
Many scientific topics are being studied using our advanced single-molecule techniques, including nucleotide-dependent assembly/disassembly dynamics of multimeric enzymes, conformation change upon complex formation, biophysical impact of phosphorylation, dilute-phase molecular organization of biological liquid condensates. We gain biophysical insights by directly monitoring these processes at the single-molecule level, which has many advantages compared to traditional ensemble-level assays.
We are always looking for enthusiastic and motivated people. Learn about open positions in our lab at the postdoc, graduate student, and postbac levels.
- Lewis-Sigler Experimental Fellow, Princeton University, 2016-2021
- Ph.D., Stanford University, 2015
- M.S., University of New Mexico, 2007
- B.S., University of Science and Technology of China, 2005
- Wilson H, Wang Q. ABEL-FRET: tether-free single-molecule FRET with hydrodynamic profiling. Nat Methods. 2021;18(7):816-820.
- Wilson H, Wang Q. Joint Detection of Change Points in Multichannel Single-Molecule Measurements. J Phys Chem B. 2021;125(49):13425-13435.
- Squires AH, Wang Q, Dahlberg PD, Moerner WE. A bottom-up perspective on photodynamics and photoprotection in light-harvesting complexes using anti-Brownian trapping. J Chem Phys. 2022;156(7):070901.
- He G, GrandPre T, Wilson H, Zhang Y, Jonikas MC, Wingreen NS, Wang Q. Phase-separating pyrenoid proteins form complexes in the dilute phase. Commun Biol. 2023;6(1):19.
Related Scientific Focus Areas
Biomedical Engineering and Biophysics
Molecular Biology and Biochemistry
This page was last updated on Thursday, November 30, 2023