Nan-Ping Peter Weng, M.D., Ph.D.

Senior Investigator

Laboratory of Molecular Biology and Immunology

NIA

251 Bayview Boulevard
Suite 100
Baltimore, MD 21224

410-558-8341

wengn@mail.nih.gov

Research Topics

Age-associated changes in the adaptive immune system are complex and highly consequential for the health and wellbeing of older humans. What we have learned for the past decades are alterations of compositions of T and B cells and their functions with advance of age. However, the mechanisms underlying these age-related changes in lymphocytes are not fully understood. Our research interests are 1) to identify the key transcriptome and epigenetic changes that causes age-related reduced functions of CD8 T cells including both naïve and memory cells. We are applying the genomic and molecular techniques and methods including RNAseq, scRNAse, ATACseq and ChIPseq to analyze human and mouse T cells; 2) to determine the size of general and antigen-specific ab TCR repertoires and their changes with age. We have incorporated tetramer staining and cell sort to isolate antigen specific CD8 T cells for their repertoire and individual TCR structure and function analyses using deep sequencing and x-ray crystal structures; and 3) to elucidate the role of telomere and telomerase in regulation of lymphocyte proliferation and function in human longitudinal follow-up samples as well as genetically modified mouse models. Collectively, we hope to elucidate the mechanisms underlying age-associated changes in T cells.

Biography

Dr. Weng received his M.D. from Fudan University Shanghai Medical College (former Shanghai First Medical College), China in 1984 and Ph.D. in Immunology from Baylor College of Medicine in 1993. He obtained his postdoctoral training at National Cancer Institute, NIH. He joined the Laboratory of Immunology at NIA as a tenure-track investigator in 1997 and was tenured in 2006.

Selected Publications

  1. Sun X, Nguyen T, Achour A, Ko A, Cifello J, Ling C, Sharma J, Hiroi T, Zhang Y, Chia CW, Wood Iii W, Wu WW, Zukley L, Phue JN, Becker KG, Shen RF, Ferrucci L, Weng NP. Longitudinal analysis reveals age-related changes in the T cell receptor repertoire of human T cell subsets. J Clin Invest. 2022.

  2. Ko A, Watanabe M, Nguyen T, Shi A, Achour A, Zhang B, Sun X, Wang Q, Zhuang Y, Weng NP, Hodes RJ. TCR Repertoires of Thymic Conventional and Regulatory T Cells: Identification and Characterization of Both Unique and Shared TCR Sequences. J Immunol. 2020;204(4):858-867.

  3. Patrick MS, Cheng NL, Kim J, An J, Dong F, Yang Q, Zou I, Weng NP. Human T Cell Differentiation Negatively Regulates Telomerase Expression Resulting in Reduced Activation-Induced Proliferation and Survival. Front Immunol. 2019;10:1993.

  4. Chen G, Subedi K, Chakraborty S, Sharov A, Lu J, Kim J, Mi X, Wersto R, Sung MH, Weng NP. Ezh2 Regulates Activation-Induced CD8+ T Cell Cycle Progression via Repressing Cdkn2a and Cdkn1c Expression. Front Immunol. 2018;9:549.

  5. Roy R, Ramamoorthy S, Shapiro BD, Kaileh M, Hernandez D, Sarantopoulou D, Arepalli S, Boller S, Singh A, Bektas A, Kim J, Moore AZ, Tanaka T, McKelvey J, Zukley L, Nguyen C, Wallace T, Dunn C, Wersto R, Wood W, Piao Y, Becker KG, Coletta C, De S, Sen JM, Battle A, Weng NP, Grosschedl R, Ferrucci L, Sen R. DNA methylation signatures reveal that distinct combinations of transcription factors specify human immune cell epigenetic identity. Immunity. 2021;54(11):2465-2480.e5.


This page was last updated on August 6th, 2022