Michael J. O'Donovan, M.D., Ph.D.
Developmental Neurobiology Section
Building 35, Room 3C-1014
35 Convent Drive
Bethesda, MD 20892-3700
We have also been using calcium and voltage-sensitive dye imaging to visualize the spatiotemporal patterns of neuronal activity associated with rhythmic locomotor-like bursting. This work has revealed the existence of a rostro-caudal 'wave' of excitation in the activation of rostral lumbar and sacral motoneurons during each cycle of rhythmic activity. We are currently using imaging the visualize the neuronal populations that are active during spinal network activity.
We are also using optogenetics to examine the role of motoneurons and other neuronal classes in locomotor rhythmogenesis and pattern formation.
Falgairolle M, O'Donovan MJ. Pharmacological Investigation of Fluoro-Gold Entry into Spinal Neurons. PLoS One. 2015;10(6):e0131430.
Pujala A, Blivis D, O'Donovan MJ. Interactions between Dorsal and Ventral Root Stimulation on the Generation of Locomotor-Like Activity in the Neonatal Mouse Spinal Cord. eNeuro. 2016;3(3).
Etlin A, Finkel E, Mor Y, O'Donovan MJ, Anglister L, Lev-Tov A. Characterization of sacral interneurons that mediate activation of locomotor pattern generators by sacrocaudal afferent input. J Neurosci. 2013;33(2):734-47.
Thirumalai V, Behrend RM, Birineni S, Liu W, Blivis D, O'Donovan MJ. Preservation of VGLUT1 synapses on ventral calbindin-immunoreactive interneurons and normal locomotor function in a mouse model of spinal muscular atrophy. J Neurophysiol. 2013;109(3):702-10.
Yoshida Y, Yoshimi R, Yoshii H, Kim D, Dey A, Xiong H, Munasinghe J, Yazawa I, O'Donovan MJ, Maximova OA, Sharma S, Zhu J, Wang H, Morse HC 3rd, Ozato K. The transcription factor IRF8 activates integrin-mediated TGF-β signaling and promotes neuroinflammation. Immunity. 2014;40(2):187-98.
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This page was last updated on January 22nd, 2014