Henry L. Levin, Ph.D.

Senior Investigator

Section on Eukaryotic Transposable Elements

NICHD/DIR

49 1A35
20892-4417

301-402-4281

henry_levin@nih.gov

Research Topics

The biological impact of transposable elements

Transposable elements were highly active throughout evolution resulting in genome landscapes dominated by sequence repeats. The regulatory sequences contained within transposable elements have been broadly dispersed to form gene regulatory networks important for many biological functions. Our current studies focus on the integration of transposable elements and how de novo insertion modifies host biology. Experimental systems include the LTR retrotransposon of fission yeast, and HIV-1 in human cells. Recent work relies on the vast databases of human genetics to understand the role of transposable elements in human disease. 

Biography

Dr. Henry Levin is the head of the Section of Eukaryotic Transposable Elements. He received his PhD from University of California, Berkeley and then was a postdoctoral fellow at Johns Hopkins University with Jef Boeke. Dr. Levin’s studies on the LTR retrotransposons in fission yeast identified mechanistic details of particle formation, reverse transcription, and integration. This research led to innovative methods of DNA sequencing that revealed integration behavior of transposable elements. With these methods the laboratory demonstrated that transposable element integration in fission yeast occurs primarily at promoters resulting in altered gene expression. These sequencing methods were also applied to studies of HIV-1 and provided new insight into the integration behavior of HIV-1. In addition, Dr. Levin develops novel technologies that rely on transposable elements to characterize genetic function genome-wide. Dr. Levin has organized several conferences and symposia on transposon biology and retrovirus replication. 

Selected Publications

  1. Chatterjee AG, Esnault C, Guo Y, Hung S, McQueen PG, Levin HL. Serial number tagging reveals a prominent sequence preference of retrotransposon integration. Nucleic Acids Res. 2014;42(13):8449-60.

  2. Guo Y, Park JM, Cui B, Humes E, Gangadharan S, Hung S, FitzGerald PC, Hoe KL, Grewal SI, Craig NL, Levin HL. Integration profiling of gene function with dense maps of transposon integration. Genetics. 2013;195(2):599-609.

  3. Feng G, Leem YE, Levin HL. Transposon integration enhances expression of stress response genes. Nucleic Acids Res. 2013;41(2):775-89.

  4. Singh PK, Plumb MR, Ferris AL, Iben JR, Wu X, Fadel HJ, Luke BT, Esnault C, Poeschla EM, Hughes SH, Kvaratskhelia M, Levin HL. LEDGF/p75 interacts with mRNA splicing factors and targets HIV-1 integration to highly spliced genes. Genes Dev. 2015;29(21):2287-97.

  5. Rai SK, Sangesland M, Lee M Jr, Esnault C, Cui Y, Chatterjee AG, Levin HL. Host factors that promote retrotransposon integration are similar in distantly related eukaryotes. PLoS Genet. 2017;13(12):e1006775.


This page was last updated on November 5th, 2018