Guang Hu, Ph.D.
Epigenetics & Stem Cell Biology Laboratory / Stem Cell Biology Group
Building 101, Room D416
111 T.W. Alexander Drive
Research Triangle Park, NC 27709
Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) can be used as a model system to study the molecular basis of fate-specification during early mammalian development. They can also be used to derive various types of cells for disease modeling, drug discovery, regenerative medicine, toxicity testing, and environmental health studies.
Successful application of ESC and iPSC to the above studies depends on a thorough understanding of the mechanisms that control their self-renewal and differentiation. We have previously carried out a genome-wide RNAi screen in mouse ESCs and identified a list of novel genes that are important self-renewal. We are currently investigating the function of several of these novel genes in ESCs and iPSCs with biochemical, genetic, and genomic approaches.
In addition to the studies of those newly-identified self-renewal factors, we are developing and applying functional and chemical genetic approaches to identify and probe new genes and networks involved in stem cell fate specification. We plan to study the differentiation of ESCs into specific lineages and the self-renewal of other types of stem cells. We also plan to study the effect of environmental chemicals on ESC and iPSC differentiation and development.
Guang Hu, Ph.D., leads the Stem Cell Biology Group within the Laboratory of Molecular Carcinogenesis. He earned his Ph.D. in 2003 at Baylor College of Medicine, Houston, Texas. Hu was a Helen Hay Whitney Foundation fellow from 2004 to 2007 and completed a postdoctoral fellowship at Harvard Medical School under Stephen Elledge, Ph.D., before joining NIEHS in 2009.
Zheng X, Dumitru R, Lackford BL, Freudenberg JM, Singh AP, Archer TK, Jothi R, Hu G. Cnot1, Cnot2, and Cnot3 maintain mouse and human ESC identity and inhibit extraembryonic differentiation. Stem Cells. 2012;30(5):910-22.
Lackford B, Yao C, Charles GM, Weng L, Zheng X, Choi EA, Xie X, Wan J, Xing Y, Freudenberg JM, Yang P, Jothi R, Hu G, Shi Y. Fip1 regulates mRNA alternative polyadenylation to promote stem cell self-renewal. EMBO J. 2014;33(8):878-89.
Wang L, Miao YL, Zheng X, Lackford B, Zhou B, Han L, Yao C, Ward JM, Burkholder A, Lipchina I, Fargo DC, Hochedlinger K, Shi Y, Williams CJ, Hu G. The THO complex regulates pluripotency gene mRNA export and controls embryonic stem cell self-renewal and somatic cell reprogramming. Cell Stem Cell. 2013;13(6):676-90.
Hu G, Kim J, Xu Q, Leng Y, Orkin SH, Elledge SJ. A genome-wide RNAi screen identifies a new transcriptional module required for self-renewal. Genes Dev. 2009;23(7):837-48.
Wang L, Du Y, Ward JM, Shimbo T, Lackford B, Zheng X, Miao YL, Zhou B, Han L, Fargo DC, Jothi R, Williams CJ, Wade PA, Hu G. INO80 facilitates pluripotency gene activation in embryonic stem cell self-renewal, reprogramming, and blastocyst development. Cell Stem Cell. 2014;14(5):575-91.