Daniel C. Masison, Ph.D.
Protein Chaperones and Amyloid Section, Laboratory of Biochemistry and Genetics
Building 8, Room 324
8 Center Drive
Bethesda, MD 20814
Our goal is to understand how protein chaperones and co-chaperones function at a molecular level. We want to understand their intrinsic functions and how they cooperate, regulate and fine-tune each other’s activities, as well as how their activities influence propagation of amyloid in cells.
We use genetics, molecular biology, and biochemistry to identify and characterize cellular factors that influence propagation of prions in yeast. The prions we study are proteins that have a propensity to misfold and form highly structured fibrous aggregates called amyloid. Tissue pathalogy in many human disorders, including type 2 diabetes and Alzheimer’s disease, is associated with accumulation of amyloid forms of proteins. Prions are transmissible between strains and they propagate by recruiting the normal form of the protein and converting it into the same misshapen form as it joins the growing fiber. Prions also must replicate to be maintained in a growing population of yeast.
Hsp70 is a universally conserved and essential protein chaperone that helps proteins adopt and maintain their native structural conformations. Hsp70 activity is regulated by co-chaperones, such as Hsp40. Another protein chaperone, Hsp104, acts with Hsp70 and Hsp40 to break up and reactivate proteins that have become aggregated. The normal function of this chaperone machine promotes amyloid propagation by breaking fibers into more numerous pieces, each of which can continue to propagate the amyloid structure. We have identified several mutations in these chaperones that variously affect the prion seeding process. Determining how these mutations affect the chaperones’ activities and ability to interact with each other will help us understand the molecular mechanisms underlying the functions of this machinery.
We have also found that TPR co-chaperones, which are components of the Hsp90 machinery, can regulate Hsp70 function in prion propagation, independently of Hsp90.We are also using our system to dissect the mechanisms of how these co-chaperones regulate Hsp70 and Hsp90 activity, and for assessing in vivo functions of Hsp90.
We have identified many mutants of various protein chaperones, in particular Hsp70 and its co-chaperones, that are revealing chaperone and co-chaperone interactions that are important for prion replication. We are continuing genetic characterization of the modified chaperones and working to define how the mutations affect their physical interactions and enzymatic activities. We are particularly interested in understanding how the various chaperones interact with each other to regulate each other’s functions and to cooperate as components of different protein remodeling machines. We also are working to understand how specific interactions among the many different components act to localize, direct, and fine-tune the chaperone machinery in its diverse cellular processes. Many of the chaperone "defects" that disrupt amyloid propagation have little or no effect on cell physiology, which points to these proteins as attractive targets for therapy in amyloid diseasses. In addition to providing a better understanding of how protein chaperones function in amyloid propagation, our continued studies will provide insight into how they act in their many important roles within the cell.
Applying our Research
By understanding the molecular interactions that influence propagation of amyloid, our work might help in developing strategies to combat it.
- Ph.D., University of Massachusetts Medical Center, 1993
- B.S., University of Massachusetts, 1983
Reidy M, Miot M, Masison DC. Prokaryotic chaperones support yeast prions and thermotolerance and define disaggregation machinery interactions. Genetics. 2012;192(1):185-93.
Sharma D, Masison DC. Single methyl group determines prion propagation and protein degradation activities of yeast heat shock protein (Hsp)-70 chaperones Ssa1p and Ssa2p. Proc Natl Acad Sci U S A. 2011;108(33):13665-70.
Reidy M, Sharma R, Shastry S, Roberts BL, Albino-Flores I, Wickner S, Masison DC. Hsp40s specify functions of Hsp104 and Hsp90 protein chaperone machines. PLoS Genet. 2014;10(10):e1004720.
Reidy M, Sharma R, Roberts BL, Masison DC. Human J-protein DnaJB6b Cures a Subset of Saccharomyces cerevisiae Prions and Selectively Blocks Assembly of Structurally Related Amyloids. J Biol Chem. 2016;291(8):4035-47.
Hung GC, Masison DC. N-terminal domain of yeast Hsp104 chaperone is dispensable for thermotolerance and prion propagation but necessary for curing prions by Hsp104 overexpression. Genetics. 2006;173(2):611-20.
Related Scientific Focus Areas
Molecular Biology and Biochemistry
Genetics and Genomics
This page was last updated on May 26th, 2016