Attila Szabo, Ph.D.
NIH Distinguished Investigator
Theoretical Biophysical Chemistry Section, Laboratory of Chemical Physics
Building 5, Room 138
5 Memorial Dr
Bethesda, MD 20814
+1 301 496 2650
The primary goal of our work is to develop the theoretical framework needed to understand the behavior of molecules at the single-molecule level. The idea is to extract quantitative information from raw experimental data (a photon trajectory or a force extension curve). This requires a complete understanding of all complex microscopic processes involved and the ability to describe them mathematically.
Our second goal is to work with leading experimental groups and show them how to correctly analyze and interpret their experiments. Our work helps them to obtain a detailed microscopic description of the specific biological processes under investigation. Our work is significant because it opens the way for single-molecule experiments that provide both qualitative and quantitative answers to questions that scientists cannot address using conventional ensemble experiments.
Our research aims to bridge the gap between theory and experiment. We are developing the theories required to analyze and interpret both single-molecule optical (where the output is a sequence of photons with different colors) and mechanical (where the output is a force-extension curve) experiments. The goal of our work is to extract both kinetic and thermodynamic information. In addition, one of our long-term interests is to understand the role of diffusion in determining the rates of chemical reactions, including ligand binding and protein-protein association.
Applying our Research
New and powerful drugs are becoming more difficult to find. Therefore, scientists need to understand biological processes in an ever-increasing amount of detail. Our work focuses on the behavior of individual molecules as they carry out their function. As with most basic research, potential practical applications may not be immediately clear, but one thing is certain: all past discoveries have been intimately linked with deeper understanding.
- External Member of the Hungarian Academy of Science, 2013
- Member of the National Academy of Sciences, 2010
- Member of the American Academy of Arts & Sciences, 2009
- Fellow of the American Physical Society, 2007
- Fellow of the Biophysical Society, 2007
- Ph.D., Harvard University, 1973
- B.S., McGill University, 1968
Cossio P, Hummer G, Szabo A. Transition paths in single-molecule force spectroscopy. J Chem Phys. 2018;148(12):123309.
Gopich IV, Szabo A. Influence of diffusion on the kinetics of multisite phosphorylation. Protein Sci. 2016;25(1):244-54.
Gopich IV, Szabo A. Theory of the energy transfer efficiency and fluorescence lifetime distribution in single-molecule FRET. Proc Natl Acad Sci U S A. 2012;109(20):7747-52.
Cossio P, Hummer G, Szabo A. On artifacts in single-molecule force spectroscopy. Proc Natl Acad Sci U S A. 2015;112(46):14248-53.
Berezhkovskii AM, Szabo A. Effect of ligand diffusion on occupancy fluctuations of cell-surface receptors. J Chem Phys. 2013;139(12):121910.
Related Scientific Focus Areas
Biomedical Engineering and Biophysics
This page was last updated on April 26th, 2021