Adrian Marcelo Zelazny, Ph.D., D(ABMM)

Senior Scientist

Department of Laboratory Medicine

NIH Clinical Center

Building 10, Room 2C385B
10 Center Drive
Bethesda, MD 20892


Research Topics

Dr. Zelazny's basic research interests include host-pathogen interactions and the evolution of mycobacteria in outbreaks and in persistent infections.


Dr. Zelazny joined the NIH Microbiology Service in 2008 following completion of the NIH ASM-CPEP-approved Postdoctoral Training Program in Clinical Microbiology and the board certification exam. He became chief of the Department of Laboratory Medicine’s Microbiology Service in 2021.

Dr. Zelazny has been the Head of the Mycobacteriology Laboratory in the Microbiology Service since 2008. He was also the director of the NIH Clinical Microbiology Fellowship program from 2012 to 2020.

The Mycobacteriology laboratory maintains a large, on-going collection of clinical isolates of mycobacteria used for patient-care and research purposes.

One major goal of the laboratory is the development of novel proteomic and genomic-based diagnostic assays. Under Dr. Zelazny’s direction, the laboratory has pioneered the use of MALDI TOF mass spectrometry for the identification of mycobacteria and fungi and developed new diagnostic assays for identification and typing of rapidly growing mycobacteria and in particular M. abscessus and more recently the evaluation of a proteomic method direct detection of mycobacteria in patient samples.

Selected Publications

  1. Li L, Henkle E, Youngquist BM, Seo S, Hamed K, Melnick D, Lyon CJ, Jiang L, Zelazny AM, Hu TY, Winthrop KL, Ning B. Serum Cell-Free DNA-based Detection of Mycobacterium avium Complex Infection. Am J Respir Crit Care Med. 2024;209(10):1246-1254.
  2. Shallom SJ, Tettelin H, Chandrasekaran P, Park IK, Agrawal S, Arora K, Sadzewicz L, Milstone AM, Aitken ML, Brown-Elliott BA, Wallace RJ Jr, Sampaio EP, Niederweis M, Olivier KN, Holland SM, Zelazny AM. Evolution of Mycobacterium abscessus in the human lung: Cumulative mutations and genomic rearrangement of porin genes in patient isolates. Virulence. 2023;14(1):2215602.
  3. Manion M, Dulanto Chiang A, Pei L, Wong CS, Khil P, Hammoud DA, Anderson M, Laidlaw E, Kuriakose S, Lisco A, NISC Comparative Sequencing Program, Zelazny AM, Dekker JP, Sereti I. Disseminated Mycobacterium marinum in Human Immunodeficiency Virus Unmasked by Immune Reconstitution Inflammatory Syndrome. J Infect Dis. 2021;224(3):453-457.
  4. Korol CB, Shallom SJ, Arora K, Boshoff HI, Freeman AF, King A, Agrawal S, Daugherty SC, Jancel T, Kabat J, Ganesan S, Torrero MN, Sampaio EP, Barry C 3rd, Holland SM, Tettelin H, Rosenzweig SD, Zelazny AM. Tissue specific diversification, virulence and immune response to Mycobacterium bovis BCG in a patient with an IFN-γ R1 deficiency. Virulence. 2020;11(1):1656-1673.

Related Scientific Focus Areas

This page was last updated on Tuesday, June 18, 2024