Yun-Xing Wang, Ph.D.
Structural Biophysics Laboratory
Building 538, Room 116
Frederick, MD 27102-1201
The long-term research interests of Dr. Wang's laboratory are to understand the fundamental interactions regulating essential events involving RNA in the translational and post-translational processes on both the structural and cellular levels using NMR spectroscopy and various other biophysical, biochemical, and biological methods. For useful resources, please go to https://ccrod.cancer.gov/confluence/display/CCRSBL1/Home
Currently, the lab focuses on the key structural element(s) in 3' and 5' prime UTR RNAs that are important for gene regulation. The lab is studying the structural basis of an adenine riboswitch, which regulates the gene expression in response to the level of adenine as a metabolite. This riboswitch is located in the 5' end of bacterial mRNA. The structural knowledge of the switching may be important to understand the fundamental mechanism of regulation of gene expression by RNA regulators.
Researchers in Dr. Wang's laboratory are also engaged in developing new methods and technologies to achieve research objectives. The Wang laboratory has been at the forefront of using small angle X-ray scattering (SAXS) to study structures and dynamics of RNAs and proteins. The laboratory has established the CCR SAXS Core Facility, which is open to all intramural and extramural research communities. For the past four years, more than 80 laboratories and research groups in the U.S. and from other countries have used the CCR SAXS resources.
Dr. Wang conducted his graduate work on structure determination of fragments of 23S rRNA using NMR spectroscopy and UV-melting experiments in Professor David E. Draper's lab of the Johns Hopkins University. Dr. Wang received his Ph.D. in October 1994 from the Johns Hopkins University. From 1994 to 2000 he was an NIH postdoctoral later a research fellow in Dr. Dennis Torchia's laboratory where he studied the structure, hydration dynamics of HIV-1 protease in complex with inhibitors and elucidated the 3D structure and a new function of the antitumor/anti-HIV protein MAP30. In the late 2000 he then joined the Structural Biophysics Laboratory of NCI. Using high field NMR spectroscopy and other biophysical and biochemical methods, his group studies the functional structural biology of RNAs and proteins.
Chang FM, Coyne HJ, Cubillas C, Vinuesa P, Fang X, Ma Z, Ma D, Helmann JD, García-de los Santos A, Wang YX, Dann CE 3rd, Giedroc DP. Cu(I)-mediated allosteric switching in a copper-sensing operon repressor (CsoR). J Biol Chem. 2014;289(27):19204-17.
Fang X, Wang J, O'Carroll IP, Mitchell M, Zuo X, Wang Y, Yu P, Liu Y, Rausch JW, Dyba MA, Kjems J, Schwieters CD, Seifert S, Winans RE, Watts NR, Stahl SJ, Wingfield PT, Byrd RA, Le Grice SF, Rein A, Wang YX. An unusual topological structure of the HIV-1 Rev response element. Cell. 2013;155(3):594-605.
Giladi M, Sasson Y, Fang X, Hiller R, Buki T, Wang YX, Hirsch JA, Khananshvili D. A common Ca2+-driven interdomain module governs eukaryotic NCX regulation. PLoS One. 2012;7(6):e39985.
Chen B, Zuo X, Wang YX, Dayie TK. Multiple conformations of SAM-II riboswitch detected with SAXS and NMR spectroscopy. Nucleic Acids Res. 2012;40(7):3117-30.
Burke JE, Sashital DG, Zuo X, Wang YX, Butcher SE. Structure of the yeast U2/U6 snRNA complex. RNA. 2012;18(4):673-83.