Masur Auditorium, NIH Clinical Center (Bldg 10)
Rachel Wong, Professor in the Department of Biological Structure at the University of Washington, will present “Assembly, disassembly and reassembly of circuits in the retina" next in the Wednesday Afternoon Lecture Series (WALS).
from the speaker: “My lab is interested in neuronal circuit assembly in development, circuit disassembly in degeneration and circuit reassembly upon cellular regeneration. Our studies are based on the vertebrate retina of zebrafish and mice. We apply a diversity of approaches including in vivo and in vitro confocal and multiphoton imaging, electron microscopy, transgenic methods and electrophysiology to investigate neuronal structure, function and connectivity in normal and perturbed retinas. We have developed and applied cellular and synaptic labels to visualize circuits in vivo and in vitro, largely through biolistics and transgenic approaches. Correlative fluorescence and serial electron microscopy enables us to map identified synaptic connections onto individual neurons. Both normal and perturbed cell function are probed using electrophysiological techniques including whole-cell and multielectrode array recordings. We are currently investigating the cellular mechanisms and developmental strategies that establish excitatory and inhibitory circuits in the mammalian retina. By taking advantage of the zebrafish’s capacity to regenerate neurons, we are also determining how newly-generated neurons integrate into existing circuitry.”