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Jennifer A. Lippincott-Schwartz, Ph.D.

NIH Distinguished Investigator
NICHD
Section on Organelle Biology
Building 32
9000 Rockville Pike
Bethesda, MD 20892
301-402-1009
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Research Topics

Dynamics of Membrane Trafficking, Sorting, and Compartmentalization Within Eukaryotic Cells

We investigate the global principles underlying secretory membrane trafficking, sorting, and compartmentalization within eukaryotic cells. We use live-cell imaging of green fluorescent protein (GFP) fusion proteins in combination with photobleaching and photoactivation techniques to investigate the subcellular localization, mobility, transport routes, and turnover of a variety of proteins with important roles in the organization and regulation of membrane trafficking and compartmentalization. To test mechanistic hypotheses about protein and organelle dynamics, we use quantitative measurements of these protein characteristics in kinetic modeling and simulation experiments. Among the topics under investigation are (i) membrane partitioning and its role in protein sorting and transport in the Golgi apparatus; (ii) biogenesis and dynamics of unconventional organelles; (iii) mitochondrial morphology and its regulation of cell cycle progression; and (iv) cytoskeletal and endomembrane cross-talk in polarized epithelial cells, hematopoietic niche cells, and the developing Drosophila syncytial blastoderm embryo. We also devoted considerable effort to developing new fluorescence microscopy techniques for imaging fluorescently tagged proteins at near-molecular resolution.

Biography

Dr. Jennifer Lippincott-Schwartz obtained her Ph.D. from Johns Hopkins University in Baltimore, MD and received postdoctoral training with Dr. Richard Klausner of NICHD. She is currently Chief of the Section on Organelle Biology in the Cell Biology and Metabolism Branch of the NICHD. Dr. Lippincott-Schwartz's research uses live cell imaging approaches to analyze the spatio-temporal behavior and dynamic interactions of molecules in cells. These approaches have helped to change the conventional “static” view of protein distribution and function in cells to a more dynamic view that integrates information on protein localization, concentration, diffusion, and interactions that are indiscernible from protein sequences and in vitro biochemical experiments alone. The projects in Lippincott-Schwartz's lab cover a vast range of cell biological topics, including protein transport and the cytoskeleton, organelle assembly and disassembly, and the generation of cell polarity. Analysis of the dynamics of fluorescently labeled proteins expressed in cells is performed using numerous live cell imaging approaches, including FRAP, FCS, and photoactivation. Most recently, her research has focused on the development and use of photoactivatable fluorescent proteins, which “switch on” in response to uv light. One application of these proteins she has put to use is in photoactivated localization microscopy (i.e., PALM), a superresolution imaging technique that enables visualization of molecule distributions at high density at the nano-scale. Dr. Lippincott-Schwartz serves as Editor for Current Protocols in Cell Biology and The Journal of Cell Science and she is on the editorial boards of Cell, Physiology, and Molecular Biology of the Cell. She is an active member of the scientific community, having served as a member of the Council for the American Society of Cell Biology and on the Executive Board of the Biophysical Society. She was elected to the National Academy of Sciences in 2008 and the National Institute of Medicine in 2009.

Selected Publications

  1. Sengupta P, Jovanovic-Talisman T, Skoko D, Renz M, Veatch SL, Lippincott-Schwartz J. Probing protein heterogeneity in the plasma membrane using PALM and pair correlation analysis. Nat Methods. 2011;8(11):969-75.
  2. Rambold AS, Kostelecky B, Elia N, Lippincott-Schwartz J. Tubular network formation protects mitochondria from autophagosomal degradation during nutrient starvation. Proc Natl Acad Sci U S A. 2011;108(25):10190-5.
  3. Burnette DT, Manley S, Sengupta P, Sougrat R, Davidson MW, Kachar B, Lippincott-Schwartz J. A role for actin arcs in the leading-edge advance of migrating cells. Nat Cell Biol. 2011;13(4):371-81.
  4. Elia N, Sougrat R, Spurlin TA, Hurley JH, Lippincott-Schwartz J. Dynamics of endosomal sorting complex required for transport (ESCRT) machinery during cytokinesis and its role in abscission. Proc Natl Acad Sci U S A. 2011;108(12):4846-51.
  5. Fu D, Wakabayashi Y, Lippincott-Schwartz J, Arias IM. Bile acid stimulates hepatocyte polarization through a cAMP-Epac-MEK-LKB1-AMPK pathway. Proc Natl Acad Sci U S A. 2011;108(4):1403-8.
This page was last updated on October 18th, 2011