Hari Shroff, Ph.D.

Senior Investigator

Section on High Resolution Optical Imaging


Building 13, Room G800
13 South Drive
Bethesda, MD 20892



Research Topics

  • Development of new imaging tools to study fast 3D cellular processes
  • Super-resolution microscopy
  • Cell motility
  • Single molecule fluorescence
  • Physical properties of highly-bent DNA molecules


    Dr. Hari Shroff received a B.S.E. in bioengineering from the University of Washington in 2001, and under the supervision of Dr. Jan Liphardt, completed his Ph.D. in biophysics at the University of California at Berkeley in 2006. He spent the next three years performing postdoctoral research under the mentorship of Eric Betzig at the Howard Hughes Medical Institute's Janelia Farm Research Campus where his research focused on development of photactivated localization microscopy (PALM), an optical superresolution technique. Dr. Shroff is now Chief of NIBIB's section on high resolution optical imaging laboratory, where he and his staff are developing new imaging tools for application in biological and clinical research.

    Selected Publications

    1. Guo M, Li Y, Su Y, Lambert T, Nogare DD, Moyle MW, Duncan LH, Ikegami R, Santella A, Rey-Suarez I, Green D, Beiriger A, Chen J, Vishwasrao H, Ganesan S, Prince V, Waters JC, Annunziata CM, Hafner M, Mohler WA, Chitnis AB, Upadhyaya A, Usdin TB, Bao Z, Colón-Ramos D, La Riviere P, Liu H, Wu Y, Shroff H. Rapid image deconvolution and multiview fusion for optical microscopy. Nat Biotechnol. 2020;38(11):1337-1346.

    2. Wu Y, Han X, Su Y, Glidewell M, Daniels JS, Liu J, Sengupta T, Rey-Suarez I, Fischer R, Patel A, Combs C, Sun J, Wu X, Christensen R, Smith C, Bao L, Sun Y, Duncan LH, Chen J, Pommier Y, Shi YB, Murphy E, Roy S, Upadhyaya A, Colón-Ramos D, La Riviere P, Shroff H. Multiview confocal super-resolution microscopy. Nature. 2021;600(7888):279-284.

    3. Christensen RP, Bokinsky A, Santella A, Wu Y, Marquina-Solis J, Guo M, Kovacevic I, Kumar A, Winter PW, Tashakkori N, McCreedy E, Liu H, McAuliffe M, Mohler W, Colón-Ramos DA, Bao Z, Shroff H. Untwisting the Caenorhabditis elegans embryo. Elife. 2015;4.

    4. York AG, Chandris P, Nogare DD, Head J, Wawrzusin P, Fischer RS, Chitnis A, Shroff H. Instant super-resolution imaging in live cells and embryos via analog image processing. Nat Methods. 2013;10(11):1122-6.

    5. Wu Y, Wawrzusin P, Senseney J, Fischer RS, Christensen R, Santella A, York AG, Winter PW, Waterman CM, Bao Z, Colón-Ramos DA, McAuliffe M, Shroff H. Spatially isotropic four-dimensional imaging with dual-view plane illumination microscopy. Nat Biotechnol. 2013;31(11):1032-8.

    This page was last updated on July 28th, 2022