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William C. Copeland, Ph.D.

Senior Investigator
Molecular Genetics Laboratory/Mitochondrial DNA Replication Group
NIEHS
Building 101, Room E316
111 T.W. Alexander Drive
Research Triangle Park, NC 27709
919-541-4792

Research Topics

Mitochondrial diseases are devastating disorders for which there is no cure and no proven treatment. About 1 in 4000 individuals is at risk of developing a mitochondrial disease sometime in their lifetime. Half of those affected are children who show symptoms before age five, and approximately 80% of them will die before age 20. The mortality rate is roughly that of cancer. The human suffering imposed by mitochondrial and metabolic diseases is enormous, yet much work is needed to understand the genetic and environmental causes of these diseases. Mitochondrial genetic diseases are characterized by alterations in the mitochondrial genome, as point mutations, deletions, rearrangements, or depletion of the mitochondrial DNA (mtDNA). The mutation rate of the mitochondrial genome is 10ñ20 times greater than of nuclear DNA, and mtDNA is more prone to oxidative damage than is nuclear DNA. Mutations in human mtDNA cause premature aging, severe neuromuscular pathologies and maternally inherited metabolic diseases, and influence apoptosis.

The primary goal of the Mitochondrial DNA Replication Group is to understand the role of DNA replication in the production and prevention of mutations in mtDNA in healthy persons and disease patients. We are investigating the proteins that encompass the mtDNA replication machinery, which include the DNA polymerase γ (encoded by the POLG gene) and its accessory subunit (POLG2), the mtDNA helicase, and the single-stranded-DNA-binding protein. Driven by its health relevance, research is focused on understanding how mitochondrial DNA replication proteins contribute to point mutations and deletions in mtDNA and how these proteins carry out mtDNA replication and repair and deal with DNA damage. We are also investigating how nucleoside reverse-transcriptase inhibitors used in AIDS therapy induce mitochondrial toxicity and why pol γ is sensitive to these analogs. Enzymology is used to ascertain the inhibition by antiviral nucleoside analogs. Finally, research is focused on understanding the consequences of disease mutations in the genes for pol γ (POLG), its accessory subunit (POLG2), and the mtDNA helicase, and how these mutant variants cause mtDNA mutagenesis and mtDNA depletion. Nearly 200 disease mutations have been identified in the POLG gene that cause deletions or depletion of mtDNA. POLG gene mutations cause Alpers-Huttenlocher syndrome, childhood myocerebrohepatopathy spectrum disorders, myoclonic epilepsy myopathy sensory ataxia, ataxia neuropathy spectrum disorders, and progressive external ophthalmoplegia with or without sensory ataxic neuropathy and dysarthria. Structure-function studies, enzymology, animal models, and single-cell eukaryotic genetic models are used to elucidate the consequences of these disease mutations.

Biography

Dr. Copeland received his Ph.D. in chemistry/biochemistry from the University of Texas at Austin in 1988. He completed his postdoctoral training at Stanford University School of Medicine, Department of Pathology studying the human DNA polymerase alpha/primase complex. He joined NIEHS in 1993. He currently heads the Mitochondrial DNA Replication Group and is Chief of the Laboratory of Molecular Genetics at the NIEHS.

Selected Publications

  1. Humble MM, Young MJ, Foley JF, Pandiri AR, Travlos GS, Copeland WC. Polg2 is essential for mammalian embryogenesis and is required for mtDNA maintenance. Hum Mol Genet. 2013;22(5):1017-25.
  2. Young MJ, Longley MJ, Li FY, Kasiviswanathan R, Wong LJ, Copeland WC. Biochemical analysis of human POLG2 variants associated with mitochondrial disease. Hum Mol Genet. 2011;20(15):3052-66.
  3. Kasiviswanathan R, Copeland WC. Ribonucleotide discrimination and reverse transcription by the human mitochondrial DNA polymerase. J Biol Chem. 2011;286(36):31490-500.
  4. Copeland WC. Inherited mitochondrial diseases of DNA replication. Annu Rev Med. 2008;59:131-46.
This page was last updated on April 10th, 2013