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Sriram Subramaniam, Ph.D.

Senior Investigator
Laboratory of Cell Biology
NCI/CCR
Building 50, Room 4306
Bethesda MD, 20892-8008
301-594-2062

Research Topics

Three-Dimensional Electron Microscopy at Molecular Resolution

For a more detailed description of our research program, please visit our lab's Web site at http://electron.nci.nih.gov.

The long-term mission of our research program is to obtain an integrated, quantitative understanding of cells and viruses at molecular resolution. We take an interdisciplinary approach to this problem by combining novel technologies for three-dimensional (3D) imaging with computational and cell biological tools. Our research efforts are presently focused on three areas: (1) determination of the structure and mechanisms underlying neutralization and cellular entry of HIV, (2) the development of automated, high-throughput workflows for structure determination of small, dynamic molecular complexes, and (3) the development of novel technologies for 3D imaging of cells and tissues, with particular emphasis on methods for understanding and diagnosing structural signatures of signal transduction and disease progression.

Biography

Dr. Sriram Subramaniam received his Ph.D. in Physical Chemistry from Stanford University and completed postdoctoral training in the Departments of Chemistry and Biology at M.I.T. He is chief of the Biophysics Section in the Laboratory of Cell Biology at the Center for Cancer Research, National Cancer Institute. He holds a visiting faculty appointment at the Johns Hopkins University School of Medicine. His current work is focused on the development of advanced technologies for imaging macromolecular assemblies using 3D electron microscopy, and their application to address fundamental problems in HIV/AIDS and cancer research.

Selected Publications

  1. Bartesaghi A, Merk A, Borgnia MJ, Milne JL, Subramaniam S. Prefusion structure of trimeric HIV-1 envelope glycoprotein determined by cryo-electron microscopy. Nat Struct Mol Biol. 2013;20(12):1352-7.
  2. Meyerson JR, Tran EE, Kuybeda O, Chen W, Dimitrov DS, Gorlani A, Verrips T, Lifson JD, Subramaniam S. Molecular structures of trimeric HIV-1 Env in complex with small antibody derivatives. Proc Natl Acad Sci U S A. 2013;110(2):513-8.
  3. Schauder DM, Kuybeda O, Zhang J, Klymko K, Bartesaghi A, Borgnia MJ, Mayer ML, Subramaniam S. Glutamate receptor desensitization is mediated by changes in quaternary structure of the ligand binding domain. Proc Natl Acad Sci U S A. 2013;110(15):5921-6.
  4. Harris AK, Meyerson JR, Matsuoka Y, Kuybeda O, Moran A, Bliss D, Das SR, Yewdell JW, Sapiro G, Subbarao K, Subramaniam S. Structure and accessibility of HA trimers on intact 2009 H1N1 pandemic influenza virus to stem region-specific neutralizing antibodies. Proc Natl Acad Sci U S A. 2013;110(12):4592-7.
  5. Earl LA, Lifson JD, Subramaniam S. Catching HIV 'in the act' with 3D electron microscopy. Trends Microbiol. 2013;21(8):397-404.
This page was last updated on May 16th, 2014