Herbert Tabor, M.D.
Laboratory of Biochemistry and Genetics
Building 8, Room 223
8 Center Drive
Bethesda, MD 20814
The polyamines putrescine, spermidine, and spermine are major polybasic compounds in all living cells. Research indicates that these amines are important for many systems related to growth and differentiation. We are interested in their physiologic functions, mechanisms of polyamine synthesis, regulation of their biosynthesis and degradation, and how they act in vivo. For this purpose, we have constructed null mutants in each of the biosynthetic steps in both Escherichia coli and in Saccharomyces cerevisiae. These mutants cannot make these amines; hence they are very useful tools to study the physiological effects due to their deprivation.
Our present research focuses on extending our studies on the biochemistry, regulation, and genetics of these amines and of the biosynthetic enzymes in S. cerevisiae and E. coli. Our work has demonstrated that the polyamines are required for growth of the organisms, their sporulation, maintenance of the killer dsRNA virus, protection against oxidative damage, protection against elevated temperatures, fidelity of protein biosynthesis, and for the maintenance of mitochondria. We have constructed clones that overproduce the various enzymes and have studied the sequence and structural characteristics of these enzymes. Our research concentrates on the structure and regulation of ornithine decarboxylase, spermidine synthase, spermine synthase, and S-adenosylmethionine decarboxylase.
Chattopadhyay MK, Chen W, Tabor H. Escherichia coli glutathionylspermidine synthetase/amidase: phylogeny and effect on regulation of gene expression. FEMS Microbiol Lett. 2013;338(2):132-40.
Chattopadhyay MK, Fernandez C, Sharma D, McPhie P, Masison DC. Yeast ornithine decarboxylase and antizyme form a 1:1 complex in vitro: purification and characterization of the inhibitory complex. Biochem Biophys Res Commun. 2011;406(2):177-82.
Chattopadhyay MK, Chen W, Poy G, Cam M, Stiles D, Tabor H. Microarray studies on the genes responsive to the addition of spermidine or spermine to a Saccharomyces cerevisiae spermidine synthase mutant. Yeast. 2009;26(10):531-44.
Chattopadhyay MK, Tabor CW, Tabor H. Polyamines are not required for aerobic growth of Escherichia coli: preparation of a strain with deletions in all of the genes for polyamine biosynthesis. J Bacteriol. 2009;191(17):5549-52.
Chattopadhyay MK, Park MH, Tabor H. Hypusine modification for growth is the major function of spermidine in Saccharomyces cerevisiae polyamine auxotrophs grown in limiting spermidine. Proc Natl Acad Sci U S A. 2008;105(18):6554-9.